INHIBITORY EFFECT OF DEXAMETHASONE AND DIPHENYLHY\| DANTOIN ON THE EXPRESSION OF GFAP AND Fos IN THE BRAIN OF EPILEPTIC RATS / 解剖学报

ABSTRACT

Objective To explore the antiepileptic effect and mechanism of glucocorticoid. Methods Animal behaviour observation and immunocytochemical staining. Results Major epilepsy was induced by pentylenetetrazole(PTZ).Dexamethasone or diphenylhydantoin(DPH) administered in rat 30?min before administration of PTZ could suppress or inhibit epileptiform.Immunocytochemistry demonstrated that there was a large number of hypertrophic astrocytes with GFAP immunoreaction in cerebral cortex,hippocampal gyrus and dentate gyrus of epileptic rats induced by PTZ.The immunoreaction of GFAP was obviously weakened,the number of positive cells was reduced,the processes were shorter and less in both groups of antiepilepsy by GC or DPH.The expression of Fos protein was in a great quantity in cerebral cortex 1 to 1^5 hours after seizure induced by PTZ,whereas their expressions were remarkable suppressed in GC or DPH antiepileptic groups. Conclusion 1^The antiepileptic effect of GC was further proved by comparing with the antiepileptic effects of DPH(a traditional antiepileptic drug). 2^Inhibited activity of astrocytes might be involved in antiepileptic mechanism of GC. 3^The change of expression of Fos protein might be closely related to epileptic actions. [