Modulation of multidrug resistance by 1-phenyl-2-palmitoylamino-3-morpholino-1-propanol in SKOV3-adriamycin-resistant cell line / 中华妇产科杂志

ABSTRACT

Objective To study the modulation of mdr1 and P glycoprotein (P gp) by 1 phenyl 2 palmitoylamino 3 morpholino 1 propanol (PPMP) in SKOV3 adriamycin resistant (SKOV3/AdrR) cell line Methods SKOV3/AdrR cells were treated with PPMP, mRNA expression of multidrug resistant (mdr1) gene was analyzed by reverse transcriptase polymerase chain reaction Intracellular rhodamine (Rh123) concentration was measured by flow cytometry Results PPMP was found to inhibit mdr1 expression of SKOV3/AdrR at the mRNA level This modulation of gene expression was content dependent and complete inhibition appeared at 25 ?mol/L PPMP treatment PPMP could increase intracellular Rh123 accumulation in SKOV3/AdrR cells After 15, 25 ?mol/L PPMP treatment, Rh123 accumulation in SKOV3/AdrR was markedly enhanced Rh123 fluorescence intensity were 389 98,426 08 respectively ( P