HSV_1-tk/GCV GENE THERAPY IN GLIOMA ANIMAL MODEL / 解剖学报
Acta Anatomica Sinica
;
(6)1957.
Article
in Chinese
| WPRIM
| ID: wpr-570075
ABSTRACT
Objective To evaluate the therapeutic effect and the mechanism of herpes simplex virus thymidine kinase Ⅰ type (HSV 1\|tk) and Ganciclovir(GCV) in animal model of human glioma. Methods We demonstrated that tk gene was stably integrated into the genome of retrovirus vector producing cell line pN 2A\|tk/VPC(VPC) by Southern blot hybridization; then we established the nude mice model of glioma by inoculating the VPC and the human glioma cells DBTRG\|05MG(05MG) at 1∶1,1∶4 ratios respectively(only inoculated 05MG cells as control group), then GCV treatment was administrated i.p.,30mg/(kg\5d), twice daily for 14 days. A week later, the nude mice were sacrificed and the pathological sections of tumor tissues were observed by light microscopy. Results The tumor mass of 1∶1 group decreased remarkably compared with 1∶4 group or control group respectively ( P
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Anatomica Sinica
Year:
1957
Type:
Article
Similar
MEDLINE
...
LILACS
LIS