Correlation between HLA-DQA1 allele and involved joints,gastrointestine and kidney in anaphylactoid purpura Han juvenile inhabited in lnner Mongolia / 中华风湿病学杂志

ABSTRACT

Objective To analyse the genetic susceptibility of HLA DQA1 allele to anaphylactoid purpura with involved joints (APIJ),gastrointestestine (APIG) and kidney (APIK) in juvenile Han inhabited in Inner Mongolia.Method There were 45 children with APIJ,39 with APIG and 32 with APIK and 90 normal children controls,all of which were proved to be no sibship and no rheumatic diseases and family history.Of them,HLA DQA1 allelic types were analysed by polymerase chain reaction sequence specific primer (PCR SSP) technique.Results Gene frequencies of HLA DQA10301 in APIJ,APIG and APIK groups (28 5%,24 9% and 25%,respectively) were significantly higher than those of control group (10 6%),and the difference had statistical significance( ? 2=12 008,7 639 and 6 882; P =0 001,0 006 and 0 009;RR=3 83,3 09 and 3 11;EF=0 36,0 295 and 0 297,respectively).In contrast,HLA DQA10302 allelic frequencies in APIJ,APIG and APIK groups (5 7%?3 9% and 6 5%,respectively) were significantly lower than those in control grorp (19%) respectively,and the difference had statistical significance ( ? 2=8 352,10 633 and 5 557; P =0 004,0 002 and 0 018;RR=0 24?0 159 and 0 272;PF=1 09?1 80 and 0 92,respectively).Conclusion The allele of HLA DQA10301 may be a susceptible gene for the Han children with APIJ,APIG and APIK in Inner Mongolia,while HLA DQA10302 gene may be the protective one.