Study on Determination Method of Gastrodine in Processed Product of Rhizoma Gastrodiae / 中成药

ABSTRACT

Objective:To determine gastrodine in the processed product of Rhizoma Gastrodiae.Methods:HPLC with Hypersil C 18 columm was used. The mobile phase was composed of acetonitrile-methanol-phosphate buffer-water(10∶15∶30∶45). The flow rate was 1mL?min -1 and detection wavelength was set at 220nm.Results:It was showed that a good linear relationship existed in the range of 0.7～3.8?g of gastrodine. Its average recovery was 97.66%. RSD was 2.2% (n=5).Conclusion:This method is quick, accurate and can be used for the determination of gastrodine of Rhizoma Gastrodiae.