Technological Improvement of Yiqi Huiyang Injection and Limited Determination of Diester Alkaloids / 广州中医药大学学报
Journal of Guangzhou University of Traditional Chinese Medicine
;
(6)2000.
Article
in Chinese
| WPRIM
| ID: wpr-570216
ABSTRACT
Objective To compare the effect of ultrafiltration method and activated carbon absorbance method on aconitine-type alkaloid (AA) content in the preparation of Yiqi Huiyang Injection (YHJ) and to evaluate the safety of YHJ by limited detrmination of diester alkaloids (DEA).Methods Acid dye colorimetry was used to detect AA content and reverse phase high performance liquid chromatography (RP-HPLC) was applied to determine the limited content of DEA. Mesaconitine, aconitine, hypaconitine were separated on a Hypersil BDS-C?18?column, with methanol and 0.05% triethylamine (70∶30) as mobile phase, wavelength at 240 nm and external standard for quantitative analysis.Results Compared with activated carbon absorbance method, the loss of AA was reduced, limited content of aconitine was within the pharmacopoeia standard method and the other two kinds of DEA were also low in YHJ prepared by ultrafiltration method.Conclusion Ultrafiltration was superior to activated carbon absorbance in the preparation of YHJ as with AA as the parameter, and the contents of DEA were very low which indicates that the toxicity of YHJ may be also low.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Guangzhou University of Traditional Chinese Medicine
Year:
2000
Type:
Article
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