Effects of aspirin on cell proliferation and apoptosis in human gastric cancer cell line SGC-7901 / 中华消化杂志

ABSTRACT

Objective To study the effect of aspirin on cell proliferation of human gastric cancer cell line SGC-7901 and the probab le mechanism involved. Methods Using MTT method, flow cytometr y (FCM),~~~3 H-TdR incorporation a nd electron microscopy, the effect of aspirin on the SGC-7901 of proliferation and related mechanism were studied. Results Results showed that th e growth of SGC -7901 was inhibited by aspirin. The inhibitory rates for growth of SGC-7901 we re positively correlated with the concentration and duration of aspirin in the m edia. ASA could inhibit DNA syntheses. Sub-G_1 pe ak was detected by FCM, and the rate of apoptosis was between 7.8% and (34.4)% . The cell ratios o f S and G_2/M increased, whereas the cell ratio of G_0/G_1 phase decreased (after) treatment and was in a dose-dependent manner. The SGC-7901 cell line exhibited some mor p hologic features of apoptosis, including cell shrinkage, nuclear condensation, D NA fragmentation, and formation of apoptotic bodies under electron microscopy. Conclusions Aspirin inhibited the growth of human gastric cancer ce ll line SGC-7901. The effects of aspirin on the phase of cell cycle and inducing apoptosis may be partially explained as the cytotoxic effects of aspirin.