One year observation on effects of estrogen, tamoxifen combined with or without fluoride on preventing bone loss in ovariectomized rats / 中华妇产科杂志

ABSTRACT

Objective To compare the effects of one year treatment of estrogen and tamoxifen, either alone or combined with fluoride on bone metabolism in ovariectomized rats Methods One hundred and forty two virgin female Sprague Dawley rats were ovariectomized (OVX) or sham operated at 6 months of age, and randomly divided into 7 groups (19-21 rats each) (1) sham operated controls; (2) OVX vehicle controls; (3)estrogen group; (4) fluoride group; (5) tamoxifen group; (6) fluoride plus estrogen; (7) fluoride plus tamoxifen The treatments lasted for one year Bone mineral density (BMD) measurement, bone histomorphometry analysis (lumbar vertebrae) and biomechanical test (3 point bending test on right femur) were performed before and after treatment The histological change of uterus were also determined at the same time Results (1) Twelve months of ovariectomy, the OVX group had significantly lower total body (279 mg/cm 2), lumbar vertebra (232 mg/cm 2) than all other groups (total body 286-298 mg/cm 2, lumber 251-266 mg/cm 2, P