The comparison among extraction methods of exosomes from T cells of rats / 重庆医学
Chongqing Medicine
;
(36): 3512-3514,3517, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-570314
ABSTRACT
Objective The purification methods of the exosomes derived form T cells were established in order to get high quan-tity exosomes .Methods Exosomes from T cells culture supernatants were purified by ExoQuick Precipitation ,ultrafiltration and sucrose gradient centrifugation ,differential ultracentrifugation ,and confirmed via using transmission electron microscopy .The pro-tein expression of the exosomes were analyzed by SDS-PAGE electrophoresis .Western blotting was used to test the expression of IL-2 .Results The protein concentration of the exosomes purified through ExoQuick Precipitation ,ultrafiltration and sucrose gradi-ent centrifugation were higher than through differential ultracentrifugation (P<0 .05) .SDS-PAGE displayed the difference among the exosome purified by three methods .Three kinds of exosomes all expressed IL-2 .Conclusion ExoQuick Precipitation ,ultrafiltra-tion and sucrose gradient centrifugation technique can obtain high purity and complete exosome sample .
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chongqing Medicine
Year:
2013
Type:
Article
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