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Time-dependenct and dose-dependenct regulation of human progesterone receptor isoforms A and B in uterine endometrial carcimoma by human insulin-like growth factor-Ⅰ / 中华妇产科杂志
Chinese Journal of Obstetrics and Gynecology ; (12)2001.
Article in Chinese | WPRIM | ID: wpr-570348
ABSTRACT
Objective We study the regulation of human progestrone receptor isoforms A and B in uterine endometrial carcinoma cell line by different concentration of human insulin like growth factor Ⅰ (IGF Ⅰ) for different time,to investigate the roles of IGF Ⅰ and progestrone receptor isoforms in uterine endometrial carcimoma Methods The uterine endometrial adenocarcinoma cell line HEC IB was cultured in vitro and the breast cancer cell line MCF 7 was used as control Western blot was applied to examine the changes of the two isoforms by different concerntration IGF Ⅰ for different time Results (1) In HEC IB cell line, 10 ng/ml IGF Ⅰ made hPRB up regulated in the first 24 h But according to lager concerntration and longer time, human progesterone receptor (hPR) B became down regulated, which were significant at 20 ng/ml IGF Ⅰ for 72 h and 40 ng/ml IGF Ⅰ for 48-72 h The change of hPRA was like hPRB (2) In MCF 7 cell line, 10 ng/ml and 40 ng/ml IGF Ⅰ made hPRA and hPRB significantly up regulated in 24, 48, 72 h Twenty ng/ml IGF Ⅰ made hPRB up regulated also in the first 24 h But in 48 h and 72 h, down regulation of hPRB was detected Twenty ng/ml IGF Ⅰ made hPRA down regulated in 24, 48, 72 h Conclusions (1) The regulation of IGF Ⅰ to hPR isoforms has cell type specific and dose dependenct and time dependenct (2) In HEC IB cell line, 10 ng/ml IGF Ⅰ made hPRB significantly up regulated in 24 h But following exposure to IGF Ⅰ at larger concentration and longer time, hPRB became down regulated The change of hPRA is like hPRB

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Obstetrics and Gynecology Year: 2001 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Obstetrics and Gynecology Year: 2001 Type: Article