Determination of five effective constituents in Compound Xinnaokang Soft Capsules by HPLC-DAD / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-570428
ABSTRACT
Objective:
To establish the method of simultaneous determination of five effective constituents tanshinose Ⅱ A、danshensu、protcate-chualdehyde、puerarin and ferulic acid.Methods:
After ultrasonic extraction, HPLC-DAD were used to determine the effective constituents on Zorbax SB-C 8 column with acetonitrile and pH3.0 water(adjusted with H 3PO 4) as a mobile phase. Under the gradient timetable the percent of acetonitrile increased from 5% to 72.5% in 45 min. The flow rate was 1.5mL?min -1 , Five constituents were detected at the most suitable detection wavelength.Results:
The average recovery of the five effective constituents is within the range of 100.1%~106.9%(n=3), The test of the accuracy within one day shows that the RSD is less than 2.2%, and the RSD among days less than 2.8%(n=3). The test solution is stable in 10h.Conclusion:
The method is accurate, simple, quick, and can be used for determining simultaneously the content of the five effective constituents in Compound Xinnaokang Soft Capsules
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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