The effects of sulindac on induction of apoptosis and expression of cyclooxygenase-2 and Bcl-2 protein in human hepatocellular carcinoma cells / 中华消化杂志

ABSTRACT

Objective To investigate the value of sulindac in chemoprevention and treatment of human hepatocellular carcinoma. Methods The hepatocellular carcinoma cell(HCC) lines (SMMC 7721 and HepG 2) were used in this study. Antiproliferation effects was measured by MTT assay, and apoptosis, cyclooxygenase 2 (COX 2) and Bcl 2 were detected by Western dot blotting. Results The growth inhibition and apoptosis of HCC cells could be induced by sulindac in a dose and time dependent manner. The effects of growth inhibition and apoptosis induction were different between 2 cell lines. After 24 hours of incubation with sulindac at the concentration of 2 mmol/L and 4 mmol/L, the expression of COX 2 and Bcl 2 were markedly reduced. Conclusions Sulindac could inhibit the growth of two HCC cell lines effectively in vitro by induction of apoptosis, which were associated with the inhibition of COX 2 and Bcl 2 expression.