Effect of fluoride on the function of osteoblast bone formation / 中华风湿病学杂志

ABSTRACT

Objective To observe the effects of different concentration of NaF on osteoblasts function of bone formation and dose effect relationship.Methods Osteoblasts were obtained from the calvaria of 24 hour newborn SD rats by enzymic degestion and cultuered in the medium with 10 -7 ～5? 10 -4 mol/L NaF.The ALP activity and osterocalcin were examined by biochemical assay and radioimmunologic assay (RIA) respectively.Results Results showed that the effect of NaF on ALP activity was in a double phase manner,e.g. low concentration NaF(10 -7 ,10 -6 ,10 -5 mol/L) increased ALP activity ( P