Determination of chlorogenic acid and baicalin in Yinhuang Oral Liquid by HPLC / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-570884
ABSTRACT
Objective:
To establish a method of HPLC for determination of chlorogenic acid and baicalin in Yinhuang Oral Liquid (Flos lonicerae, Radix Scutellariae).Methods:
LUNA C18(2) column was used. The acetonitrile 0.1% phosphoric acid solution was used as the mobile phase. The flow rate was 1.0mL?min -1 . The detection wavelength was set as 318nm.Results:
For chlorogenic acid, the linear range was 0.2568 ~ 2.0544 ?g, and the average recovery rate was 97.7% with RSD =0.8%. For baicalin, the linear range was 0.9896 ~7.9168?g, and the average recovery rate was 98.0% with RSD =0.9%.Conclusion:
The method is simple, reliable, accurate and can be applied to the quality control of the preparation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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