Determination of the contents of aristolochic acid A in Zhushalian capsule by HPLC / 重庆医科大学学报

ABSTRACT

Objective: To determine the contents of aristolochic acid A in Zhushalian capsules. Methods: Column hypersil C18 (250mm ? 4.6mm,5?m). Mobile phaseSolution of methanol - water - glacial acetic acid (79 29 1). Detection wavelength 315nm. Flow rate 1.0ml/min. Results: Aristolochic acid A was completely separated and no interference was found. The recovery rate was 98. 34 % . RSD was 0.04 % ( n = 5 ) . Conclusion: This method is simple, sensitive, accurate and reliable.