A study of TGF-?1 and bFGF in dilated cadiomyopathy by using immunohistochemistry / 重庆医科大学学报

ABSTRACT

Objective:The aim of the present study is to investigate the expression of TGF- ?1 and bFGF in human DCM myocardium, as well as the roles of TGF - ?1 and bFGF in DCM myocardium remodeling and the mechanism of sudden death induced by DCM. Methods:30 specimens of DCM heart and control have been collected from forensic pathologic autopsy. Tissues have been prepared by paraffin section for HE staining, and immunohistochemistry with antibodies against TGF?1, TGF?R1, bFGF, bFGFR. The staining results have been observed with light microscope and analyzed by image processing system. Results:The results showed that the protein expression of the TGF - ?1in DCM myocardium was increased,and there was no significant difference of the bFGF protein expression between DCM and controls. Conclusion:The results of this experiment indicate that TGF- ?1 is the most important cellular factor in DCM myocardium remodeling,and the role of bFGF in the remodeling of DCM myocardium needs to be further studied.