The effect of mild hypothermia on dynamic balance of amino acids and free radicals during cerebral ischemia and reperfusion in rats / 中华物理医学与康复杂志

ABSTRACT

Objective To evaluate the effect of mild hypothermia on amino acids and free radicals in rats during cerebral ischemia and reperfusion. Methods Sixty-three Wistar rats were used in this experiment. Zea-Longa' s method was employed to establish the middle cerebral artery occlusion model in 56 rats, which were then randomized into a normal temperature group (n=28) and a mild hypothermia group (n=28). The other 7 rats underwent sham operation and served as control. The rats in the former two groups were observed at 4 time points (3 hours of cerebral ischemia, 1, 2 and 3 hours reperfusion after 3 hours of ischemia) with regard to the dynamic changes of various amino acids and free radicals in the cortex of the rats. Results Compared with the sham operation group, the concentration of SOD and GSH-PX in the ischemic cortex of normal temperature group and mild hypothermia group decreased significantly (P