Effects of ginsenosides on glucocorticoid receptor in hemorrhagic shock rats / 中草药

ABSTRACT

Object To evaluate the effects of ginsenosides (GSS) in stem and leaves of ginseng on glucocorticoid receptor (GR) in the hemorrhagic shock rats, and study the mechanism. Methods Rats were divided into hemorrhagic shock group and control group. The rats in hemorrhagic shock groups were ig 200, 100, 50 mg/kg/d GSS, model group and control group were ig distilled water 2 mL for 10 days. The Rs of GR in brain and hepatic cytosol of rats were measured by radioligand binding assay, using [ 3H] dexamethasone as the ligand. The level of GR mRNA expression in hepatic cytosol were determined by RT-PCR. Plasma adrenocorticotrophic hormone (ACTH) and glucocorticoid (GC) concentrations were determined by the radioimmunoassay. Results Rs of GR in brain and hepatic cytosol were higher in hemorrhagic shock+GSS groups than those in hemorrhagic shock group, and the Rs of GR was the highest in hemorrhagic shock+10 mg/mL GSS group (P