Determination of cannabidiol in hemp seed oil by HPLC / 中草药
Chinese Traditional and Herbal Drugs
;
(24)1994.
Article
in Chinese
| WPRIM
| ID: wpr-571085
ABSTRACT
Object To develop the analysis method to determine the content of cannabidiol in the hemp seed oil by HPLC. Methods The chromatographic condition was Irregular-H-C 18 column (250 mm? 4.6 mm, 10 ?m). A mixture of methanol-acetonitrile-water-acetic acid (25∶50∶25∶0.4) was used as the mobile phase with a flow rate of 0.8 mL/min and the detection wavelength was 220 nm at room temperature. Results The calibration curve for cannabidiol showed good linear correlation within the concentration range of 1.2 — 9.6 ?g/mL (r=0.999 4). The average recovery and RSD was 94.6% and 1.9% (n=9) respectively. Conclusion The method is convenient, reliable and with good reappearance.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
1994
Type:
Article
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