Determination of ginsenoside Re、Rg1 in Yianhuisheng Oral Liquor by HPLC / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-571105
ABSTRACT
Objective:
To establish a HPLC method for the determination of ginsenoside Re、Rg1 in Yianhuisheng Oral Liquor(Radix Ginseng Rubra, Radix Aconiti Lateralis Preparata, Scorpio etc.)Methods:
RP-HPLC was used to quantitative analysis. The Synchropak C 4 column (250mm?4.6mm, 5?m) was used, and mobile phase was composed of acetonitrile-0.05% H 3PO 4(18∶82). Detection wavelength was at 203nm, the flow rate was 1.0 mL?min -1 , the injection volume of sample was 10?L and of reference 8?L.Results:
The linear response range from 0.432 ~3.456?g of Re(r=0.9992, n=5), 0.360~2.88?g of Rg1(r=0.9998, n=5), respectively. The recovery of Re 97.0%, RSD=1.4%, the recovery of Rg1 96.8%, RSD=1.1%, respectively.Conclusion:
The method was proved to be simple, rapid, accurate and reproducible, and suitable for content determination of the oral liquor.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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