Studies on HPLC Fingerprint of several kinds of Huanglianjiedu Decoction / 中成药

ABSTRACT

Objective:To identify Huanglianjiedu Decoction preparation (Rhizoma Coptidis, Radix Scutellariae, Cortex Phellodendri, Fructus Gardeniae) made from different technics and Japanese Kampo with fingerprint chromatographic. Methods: The HPLC method was used, chromatography conditions were C 18V column, binary solvent-delivery gradient elution, diode-array detector, internal standard (paeoniflorin) were taken as the criteria for quality control. Results: The result showed that 15～16 characteristic peaks were common, but the new technic preparation and EtOH extract showed a specific characteristic peak. And significant deviation has been found from peak-height ratio of characteristic peaks to internal standard in various preparations, which were made from different technics or from different materials. Conclusion: The good reproducibility and stability are showed in experiment results. The HPLC fingerprint can be used for identifying this traditional Chinese medicine preparations which were made from different technics or from different materials.