The Effect of Preserving Platelet Concentrate with Acidified Glucose Nutrient Solution / 中国输血杂志

ABSTRACT

The effect of preserving the platelet concentrate prepared from whole blood in domestic PVC plastic bags, using a new platelet-preserving addition——acidified glucose nutrient solution(AGN), in the condition of horizontal rotation at 22℃ was studied. The results of study showed that the platelet concentrate stored in the AGN solution for five days has its platelet counts, pH value, aggregability and hypotonic shock response (HSR) even more better than those of the control platelet concentrate preserved in the normal ACD solution. In addition, the platelet concentrate incubated with fresh plasma for two hours has its aggresability and hypotonic shock response obviously recovered. The observation of the platelet concentrate under transmission electron microscope indicated that the platelets preserved in AGN solution kept their morphology completed, without any pseudopodium. And so the effect of preserving the platelet concentrate with AGN solution in domestic PVC plastic bas is simiar to that obtained by foreign laboratories with CPD solution in the platelet preserving plastic bags of second generation.