Antisense phosphorothioate oligodeoxynucleotide resistance to respiratory syncytial virus infection in vitro / 重庆医科大学学报

ABSTRACT

Objective:To investigate the antiviral effects of antisense phosphorothioate oligodeoxynucleotide (AODN) in 9HTE infected with respiratory syncytial virus (RSV)in vitro.Methods:In this study,the antisense phosphorothioate oligodeoxynucleotide which complemented to genomic NS1 and M2mRNA of RSV was used to investigate antiviral activity in vitro.The cytopathic effect was observed and the cell survival rates were measured by MTT assay.Results:1.9HTE cell infected with RSV almost all died when the multiplicity of infection(moi)is above 0.1 after 5 days cell culture.2 The antisense phosphorothioate oligodeoxynucleotide could increase the cell survival rates in a dose dependent manner.ConclutionThese studies indicate that the genomic NS1 gene and M2mRNA may play an important role in regulating RSV replication and AODN may have inhibitory activity on RSV replication.The results established the basis for further study on new drugs against RSV infection.