Extraction of Prekallikrein Activator / 中国输血杂志

ABSTRACT

The FactorⅫ(FXII or a-Ⅻa)in Cohn's FIV was adsorbed by diato- mite,and then the FXⅡ(or ?-Ⅻa) was activated into ?-Ⅻa(Hageman factor fragment,PKA,MW31000)by the co-adsorbed kallikrein with sub- sequent releasing it toabuffer solu- tion.Finally,the product was purified by DEAE-Sephadex A_(50).The activity of prepared PKA was 8 963IU/ml,and the preparation can lead to signifi- cant reduction in blood pressure in rats.