Comparison of Anti-mycobacterial Drug Susceptibility Test Results by Institutes and Methods / 대한임상미생물학회지
Korean Journal of Clinical Microbiology
;
: 43-48, 2008.
Article
in Korean
| WPRIM
| ID: wpr-57139
ABSTRACT
BACKGROUND:
The purposes of the current study were to evaluate the concordant rates of anti-mycobacterial drug susceptibility test (DST) results in different solid media performed in different institutes, and to determine reliable susceptible testing methods.METHODS:
One hundred and twenty two Mycobacterium tuberculosis strains were isolated from patients in A Hospital in 2005. DSTs were performed by the absolute concentration method using L?wenstein Jensen medium in both A Hospital (method A-1) and B Institute (method B-1) and by the proportion method using Middlebrook 7H10 agar in B Institute (method B-2). Nine drugs were used including isoniazid and rifampin. Sensitivity and specificity of each method were estimated by using the acceptable standard of 90% for isoniazid and rifampin and 80% for other drugs. The therapeutic outcomes of quinolone-administered patients were evaluated according to ofloxacin susceptibility results.RESULTS:
Method B-1 showed sensitivity and specificity levels over the acceptable standard levels for all drugs. Method B-2 showed specificity lower than the acceptable levels for rifampin and cycloserine. Method A-1 showed specificity lower than the acceptable levels for isoniazid, streptomycin, p-aminosalicylic acid, and ofloxacin and sensitivity lower than the acceptable levels for prothionamide and cycloserine. The concordance rates of therapeutic outcomes with method B-1, method B-2, and method A-1 were 77%, 74%, and 65%, respectively.CONCLUSION:
The drug susceptibility results for some drugs were discordant between the testing laboratories and media, requiring an urgent application of quality control programs to raise the reliability of anti-mycobacterial DST.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aminosalicylic Acid
/
Prothionamide
/
Quality Control
/
Rifampin
/
Streptomycin
/
Ofloxacin
/
Sensitivity and Specificity
/
Culture Media
/
Cycloserine
/
Agar
Type of study:
Diagnostic study
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Clinical Microbiology
Year:
2008
Type:
Article
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