Study on the expressions of NF?B,IL-6 and PPARs with treatment of different oxidized Iipoproteins / 重庆医科大学学报

ABSTRACT

Objective:To investigate the expressions of NF?B and IL-6 in vascular endothelial cells with treatment of different oxidized lipoproteins; the different expressions of PPAR?, PPAR? and NF?B when incubated with oxidized lipoproteins.Methods:HUVECs were isolated from fresh umbilical cords obtained at normal deliveries.THP-1 cell line was commercially purchased. The lipoproteins were obtained by density-gradient ultracentrifugation and were oxidized through incubation with 15?mol/L CuSO 4. The different response extent of transcription factors and cytokine factors among four different oxidized lipoproteins were observed through immunohistochcmistry,FACS,and ELISA.The would-be unbalanced expressions of three transcription factors PPAR?,PPAR? and NF?B were confirmed by immunohistochemistry and FACS.Results:There were some differences among the four kinds of oxidized lipoproteins to activate the transcription factor and inflammatory factors in the EC and THP.oxLp(a) had the strongest effect.The proinflammation transcription factor NF?B has been far more vigorously activated than PPARs when encountered with oxidized lipoproteins. Their expressions were all enhanced with time.Conclusion:The different patterns of lipoproteins vary in activating the nuclear transcription factors and the inflammation factors. Unbalanced activation between PPARs and NF?B exists with the incubation of oxidized lipoproteins in vascular endothelial cells.