Effect of total saponins of Panax ginseng on K562 cells in expressing clusters of differentiation antigen and hematopoietic growth factor recepters / 重庆医科大学学报

ABSTRACT

Objective:To clarify further the mechanisms for total saponins of Panax ginseng(TSPG)in inducing differentiation of K562 cells,and to provide the theoretical basis and the experimental evidence of its clinical application.Methods:By using cell culture in vitro,morphological observation and flow cytometry,the effect of TSPG on K562 cells in expressing CD 15 ,HIR 2,EPO-R and GM-CSF-R were studied.Results:The results indicated that TSPG could induce the differentiation of K562 cells toward proerythroid cells and progranulocytic cells.It was also shown that after induction by TSPG,the ratio of positive K562 cells expressing CD 15 ,HIR 2,erythropoietin recepter(EPO-R) and granulocyte-macrophage colony stimulating facter recepter(GM-CSF-R) increased.Conclusion:K562 cells are abnormal cells whose differentiation are blocked,but its biological features are similar to the normal hematopoietic stem cells.The mechanism for TSPG inducing differentiation of K562 cells may be related to the higher expression of EPO-R and GM-CSF-R.