Antithrombotic effects of Veratrum nigrum var.ussuriense alkaloids / 中草药

ABSTRACT

Object To investigate antithrombotic effects of Veratrum nigrum L.var ussuriense Nakai alkaloids ( Vn A) in rats. Methods The electrically induced rat carotid artery thrombosis model was used to examine the antiarterial thrombosis effect of Vn A using occlusion time( OT) of carotid artery as phar- macologic index;the stasis- induced inferior vena cava thrombosis model was used to evaluate antivenous thrombosis effectof Vn A in terms of thrombosis formation rate and decrease in thrombus dry weight.Re- sults The iv administration of Vn A( 7.2— 42 .9) ?g/kg to rats resulted in a dose- dependent effect and significantprolongation in OT. Vn A at the dose of 30?g/kg produced an antiarterial thrombosis effecte- qual to thatof LAS( 1 8.0 mg/kg) .Also,a single bolus iv Vn A( 30 ?g/kg) increased OT in a time- depen- dentmanner;the antiarterial thrombosis effectwas rapid in onsetand lasted atleast80 min,and peaked at 1 5 min postdosing.Vn A( 1 5— 45 ?g/kg iv) decreased the thrombus dry weight significantly and dose- de- pendently. Conclusion Vn A has powerful inhibitory effects againstboth arterial and venous thrombosis in rats and acts in a dose- and time- dependentmanner.Its effective dose is as low as?g perkg body weightof rats.The finding of the Vn A antithrombotic effeets reveal a bright future of its R &D.