Determination of phellodenrine in Cortex Phellodendri by HPLC / 中成药
Chinese Traditional Patent Medicine
; (12)1992.
Article
in Zh
| WPRIM
| ID: wpr-571641
Responsible library:
WPRO
ABSTRACT
Objective: To determine phellodendrine in Cortex Phellodendri by HPLC. Methods : HPLC condition consists of C 18 column (Phenomenex, 150mm, 5?), actonitrile:250mL of 0.05mol?L -1 phosphoric acid +0.4mL of diethylamine (9∶91, v/v) as mobile phase, detection wavelength at 284nm. Results : The averagy recovery of phellofendrine was 101.43% ( RSD =1.56%, n =5) and the linear range of phellodrine was 0.4368~2.1840?g??L -1 , r =0.9998. Conclusion : The method is simple, accurate and reproducible, and can be used for the determination of phellodendrine in Cordex Phellodendri.
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Language:
Zh
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article