Determination of the Rhoifolin in Exocarpium Citri Grandis By RP-HPLC / 中药新药与临床药理

ABSTRACT

Objective To establish a method for the determination of Rhoifolin content in Exocarpium citri grandis (ECG). Methods The RP-HPLC method was operated on MACHEREY-NAGEL 100-5 C_(18), with methanol and wateracetic acid (61 4) as mobile phase, gradient elution, flow rate at 1.0 mL/min, and detection wavelength at 345nm. Results In the range from 0.816 ?g to 13. 056?g, Rhoifolin is in a good linearity with chromatographic peaks area, r=0.9995, the average recovery ratio is 101.064%, RSD=3.10%(n=5). The content of Rhoifolin in Tomentulosus Exocarpium Citri Grandis(TECG) ranged from 1.33% to 0.177% and that in Glabrous Exocarpium Citri Grandis (GECG) is less than 0.17%. Conclusions RP-HPLC method is with accuracy and a good reproducibility for the determination of the Rhoifolin content in ECG; the difference of Rhoifolin content in TECG and GECG is significant.