Cloning and expressing of vacA gene related segment with vacuolating activity of Helicobacter pylori / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2003.
Article
in Chinese
| WPRIM
| ID: wpr-571699
ABSTRACT
Objective:
To obtain the antigen of recombinant vacuolating segment of Helicobacter pylori vacuolating cytotoxing by gene-engineering.Methods:
The objective gene was cloned into PQE-30,sequenced and expressed using PCR and recombinant techniques.After expression,the antigenicity of recombinant protein was analyzed by Western blotting.Results:
DNA sequence analysis identified that the sequence was integrated.Compared with standard strain AF361700,1.5% of the cloned gene was mutated,one amino acid radicle was changed.SDS-PAGE revealed the molecular weight of objective recombinant protein was 27 000 as it was predicted;the level of expression product was over 30%.Western blotting results showed the objective protein could be recognized by anti-serum against HP.Conclusion:
This recombinant protein has perfect antigenicity,so it may be used as a protective antigen in preventing HP infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
2003
Type:
Article
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