Preparation Method for Reference Substances of Hyperin and Isorhamnetin-3-O-galactoside / 中药新药与临床药理
Traditional Chinese Drug Research & Clinical Pharmacology
;
(6)1993.
Article
in Chinese
| WPRIM
| ID: wpr-571893
ABSTRACT
Objective To establish a method for preparing the reference substances of hyperin and isorhamnetin- 3- O- galactoside. Methods Methanol- extract of Fructus Evodia was isolated and purified by combining solvent extraction with column chromatography and recrystallization. Hyperin and isorhamnetin- 3- O- galactoside were identified by mass spectrometry (MS), 1H nuclear magnetic resonance (1HNMR ) and 13C nuclear magnetic resonance (13CNMR) and their content was determined by HPLC. Results The purity was 99.6 % and 98.0 % for hyperin and isorhamnetin- 3- O- galactoside respectively. Conclusion This method is simple cancl effective to yield high- purity products. And these high- purity products can be used as reference substances for the research of herbal medicine.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Traditional Chinese Drug Research & Clinical Pharmacology
Year:
1993
Type:
Article
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