Determination of dihydromyricetin in different parts of Ampelopsis grossedentata in different seasons by HPLC / 中成药

ABSTRACT

AIM: To establish the determination of dihydromyricetin in stem,leaf of Ampelopsis grossedentata in different seasons from different habitat. METHODS: Novapak C_ 18 column (150mm?4.6mm,5?m) was selected as separation column at 25 ?C . Methanol-water-phosphoric acid (27∶73∶0.1) was used as a mobile phase at a flow rate of 1.0mL?min -1 . The peak of dihydromyricetin was detected at UV 290nm. RESULTS: The linearity of this method was good. The average recovery was 99.47%,RSD was 1.68%. The content of dihydromyricetin in leaf of Ampelopsis grossedentata collected in May was the highest and was three ～ four times the size of that in stem. CONCLUSIONS:The method is convient with a good separating degree and is useful basis for the develoment and utilization of Ampelopsis grossedentata.