Antagonistic effect of scutellarin on toxicity of selenium in human liver cells L-02 / 中草药

ABSTRACT

Object To investigate the antagonistic effect of scutellarin on the toxicity of selenium in human liver cells L-02. Methods MTT method was used to observe the effects of scutellarin and selenium on cell growth. The morphological changes of cells were observed by fluorescent microscope. The MDA level, thiol content, and total antioxidative capacity of cells were also measured. Results The optimum condition for scutellarin to promote cell growth was 0.5 mmol/L in 48 h culture period. Comparing with the only selenium treated group, 0.5 mmol/L scutellarin significantly antagonized the inhibition of cell growth caused by 1 and 5 ?mol/L selenium, suppressed the selenium-induced cell damage and the increase of MDA content, as well as increased the thiol content and cell antioxidation capacity. Conclusion Scutellarin markedly antagonizes the toxicity of selenium in human liver cells L-02 by inhibiting lipid peroxidation and promoting cellular antioxidation capacity.