The Purification and Immunogenicity of TB-14 Recombinant Protein of Mycobacterium tuberculosis / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
;
: 239-247, 2006.
Article
in Korean
| WPRIM
| ID: wpr-57210
ABSTRACT
BACKGROUND:
Culture filtrate proteins secreted by mycobacteria are thought to play an important role in inducing protective immunity and to develop new methods for diagnosing tuberculosis.METHODS:
A culture filtrate protein of M. avium that was strongly reactive with goat antiserum against M. intracellulare was constructed. Its homologous protein (TB-14) in M. tuberculosis was cloned, expressed and purified. The inductions of IFN-gamma stimulated with 10 microgram of TB-14 recombinant protein and 10 microgram PPD were estimated by using whole bloods from seven PPD (-) subjects, seven PPD (+) healthy volunteers and nine tuberculosis patients.RESULTS:
M. avium culture filtrate protein was confirmed as a hypothetical protein that was termed contig 116. A novel 14-kDa recombinant protein (TB-14) of M. tuberculosis was composed of 148 amino acids, including 30 amino acids of the signal peptide, and it showed 78% homology with M. avium. In the PPD (+) healthy volunteers, recombinant TB-14 protein strongly induced the secretion of IFN-gamma in whole blood cultures.CONCLUSION:
These results suggest that TB-14 recombinant protein might play an important role in inducing cell-mediated immunity against tuberculosis. Furthermore, TB-14 protein antigen and its antiserum will be available for the development of new diagnostic tools for tuberculosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tuberculosis
/
Protein Sorting Signals
/
Goats
/
Clone Cells
/
Healthy Volunteers
/
Amino Acids
/
Immunity, Cellular
/
Mycobacterium
/
Mycobacterium tuberculosis
Limits:
Humans
Language:
Korean
Journal:
Tuberculosis and Respiratory Diseases
Year:
2006
Type:
Article
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