Active Immunization Study of Colon Cancer Derived 1-8D Peptide in HHD Mice
Immune Network
; : 157-162, 2005.
Article
in En
| WPRIM
| ID: wpr-57218
Responsible library:
WPRO
ABSTRACT
BACKGROUND: 1-8D gene is a member of human 1-8 interferon inducible gene family and was shown to be overexpressed in fresh colon cancer tissues. Three peptides 1-6, 3-5 and 3-7 derived from human 1-8D gene were shown to have immunogenicity against colon cancer. METHODS: To study tumor immunotherapy of three peptides we established an active immunization model using HHD mice. D(b-/-) x beta2 microglobulin (beta2 m) null mice transgenic for a chimeric HLA-A2.1/D(b-)beta2 m single chain (HHD mice) were challenged with B16/HHD/1-8D tumor cells and were immunized with irradiated peptide-loaded RMA- S/HHD/B7.1 transfectants. In therapy model tumor growth was retarded in HHD mice that were injected with 3-5 peptide-loaded RMA-S/HHD/B7.1. In survival test vaccination with 1-8D-derived peptide protects HHD mice from tumor progression after tumor challenge. RESULTS: These studies show that peptide 3-5 derived from 1-8D gene can be the most effective candidate for the vaccine of immunotherapy against colon cancer and highlight 1-8D gene as putative colon carcinoma associated antigens. CONCLUSION: We demonstrated that RMA-S/HHD/ B7.1 loaded with 1-8D peptides, especially 3-5, immunization generates potent antitumor immunity against tumor cells in HHD mice and designed active immunization as proper immunotherapeutic protocols.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Peptides
/
Antigens, Tumor-Associated, Carbohydrate
/
Interferons
/
Immunization
/
Vaccination
/
Colon
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Colonic Neoplasms
/
Immunotherapy
Type of study:
Guideline
Limits:
Animals
/
Humans
Language:
En
Journal:
Immune Network
Year:
2005
Type:
Article