Protection of crocetin on primary culture cardiac myocyte injured by hydroxyl free radicals / 中草药

ABSTRACT

Object To study the cardio-protective effect of crocetin on primary culture cardiac myocyte injured by hydroxyl free radicals. Methods After adding crocetin, primary culture cardiac myocyte was injured by 0.5 mmol/L hydroxyl free radicals which were generated by Fenton systems. The activity of lactic dehydrogenase (LDH) and mitochondrion succinic dehydrogenase (MSD) was observed. Mitochondrion membrane potential (MMP) was detected by Rh123. The ratio of cardiac myocyte apoptosis was investigated by flow cytometry and DNA ladder electrophoresis. Results Compared with the model group, crocetin significantly decreased the activity of LDH in culture supernatant and the ratio of cardiac myocyte apoptosis, markedly increased the activity of MSD and MMP. Conclusion Crocetin has the protective effect on the apoptosis of cardiac myocyte injured by hydroxyl free radical.