Nuclear Factor-kappa B Activation and Chemokine Genes Expression in HT-29 Intestinal Epithelial Cells in Response to Clostridium difficile Toxin A Stimulation
Journal of Bacteriology and Virology
;
: 217-226, 2005.
Article
in Korean
| WPRIM
| ID: wpr-57228
ABSTRACT
Intestinal epithelial cells are known to up-regulate the expression of several chemokines in response to bacterial toxins. Since there has been little understanding on the cellular mechanisms of C. difficile toxin A-induced mucosal inflammation, we investigated whether nuclear factor-kappa B (NF-kappaB) could regulate chemokine gene expression in HT-29 intestinal epithelial cells stimulated with C. difficile toxin A. C. difficile toxin A rapidly increased signals of NF-kappaB composed with p65 and p50 subunits in HT-29 cells, whereas it decreased the signals of IkappaBalpha. Blocking the NF-kB activation by transfection with dominant negative I kappa B alpha-containing retrovirus attenuated the upregulated expression of IL-8, GRO-alpha, and MCP-1 induced by C. difficile toxin A. These results suggest that NF-kappaB is a major regulator of chemokine gene expression in C. difficile toxin A-stimulated intestinal epithelial cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Retroviridae
/
Bacterial Toxins
/
Transfection
/
Gene Expression
/
Clostridioides difficile
/
Interleukin-8
/
NF-kappa B
/
Clostridium
/
HT29 Cells
/
Chemokines
Limits:
Humans
Language:
Korean
Journal:
Journal of Bacteriology and Virology
Year:
2005
Type:
Article
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