Inhibition on growth and proliferation of human hepatoma cell lines QGY and HepG2 treated by 10-HCPT / 重庆医科大学学报

ABSTRACT

Objective:To investigate the effects of 10-HCPT on the growth and proliferation of human hepatoma cell lines QGY and HepG2 in vitro.Methods:QGY and HepG2 were cultured in RPMI1640.They were treated by different concentrations of 10-HCPT for 48 hours,and by a dose of 100?g/ml of 10-HCPT for different hours.The cell growth indexes (GI) were detected by MTT colorimetric assay.The clone formation rates of QGY and HepG2 were observed by soft agar assay.Results:The GI of HepG2 and QGY cells treated by 10-HCPT with concentration 10～360?g ?g/ml and 30～360?g ?g/ml were significantly lower than that of control groups respectively ( P 0.05).The clone formation rates of QGY and HepG2 of experimental groups were significantly lower than that of control groups respectively ( P