Determination of spinosin and ferulic acid in Suanzaoren Decoction by RP-HPLC / 中草药

ABSTRACT

Object To develop an RP-HPLC method for determination of spinosin and ferulic acid in Suanzaoren Decoction (SZRD). Methods Hypersil C 18 column was used. The mobile phase consisted of acetonitrile-water with 1% glacial acetic acid (17∶83) for spinosin and (16∶84) for ferulic acid. Detection wavelengths were set at 334 and 320 nm, respectively. The internal standard was p-hydroxybenzaldehyde for ferulic acid. Results The linear ranges for spinosin and ferulic acid were 0.960—13.44 ?g/mL (r=0.999 8) and 2.04—27.2 ?g/mL (r=0.999 7), respectively. The recoveries for them were 98.4% and 98.8%, respectively. The RSD were 2.5% and 2.8%, respectively. Conclusion This method is simple, rapids and accurate, and can be used for the determination of spinosin and ferulic acid in SZRD.