Over-expression and purification of recombinant spike protein fragment of SARS coronavirus / 重庆医科大学学报
Journal of Chongqing Medical University
; (12)1986.
Article
in Zh
| WPRIM
| ID: wpr-572444
Responsible library:
WPRO
ABSTRACT
Objective:To obtain the spike glycoprotein(S protein) of SARS coronavirus (SARS-Cov) for detection of the corresponding antibody and preparation of diagnostic kits.Methods:Genes encoding S protein were picked out to form a new gene and divided into two parts according to the prediction of antigenicty.They were obtained by nucleotide synthesis and were cloned into expression vector pET32a(+) respectively.Proteins then were induced by IPTG and purified through Ni-NTA.Results:Target proteins were successfully expressed and purified.Conclusion:The recombinant S proteins of SARS-CoV could be of great help in diagnosing the disease and investigating the virus further.
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WPRIM
Language:
Zh
Journal:
Journal of Chongqing Medical University
Year:
1986
Type:
Article