Determination of astragaloside Ⅳ in Qiluyishen Tabletes by HPLC-ELSD / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-572476
ABSTRACT
AIM:
To establish a method for the determination of Astragaloside Ⅳ in Oiluyishen Tabletes(Radix Astragali, Herba Pyrolae, Rhizoma Atractylodis Macrocephalae, Poria, Radix Codonopsis,etc.) by HPLC-ELSD.METHODS:
Chromatography was performed on a 5?m HIQsilC 18 column(4.6mm?250mm) at 25℃. The mobile phase composition was acetonitrile-water(34∶66). The flow rate was at 1.0mL?min -1. An evaporation light-scattering detector (ELSD) was used as detector with gas flow rate of 2.6 mL?min -1 and drift tube temperature at 100℃.RESULTS:
The linear range of Astragaloside Ⅳ was in the range of 2.288~ 22.88?g, r=0.999, the average recovery was 98.8% and RSD was 1.2%.CONCLUSION:
This method is simple, feasible and reproducible. It can be used for the quality control of Oiluyishen Tabletes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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