A Pharmacodynamic Study of Compound Hypericum Perforatum Granule / 中药新药与临床药理

ABSTRACT

Objective To investigate the pharmacological actions of Compound Hypericum Perforutum granule(CHPG). Methods Duck models with hepatitis B induced by 2.2.15 cell strain were used to observe the effect of CHPG on the replication of hepatitis B virus(HBV).Mice models of liver injury induced by CCl4,D-aminogalactose and BCG+LPS were applied to observe the protective effect of CHPG on liver and mice immune function.Solid-phase radioimmuoassay, spot-hybridism radiation self-developing method and MTT method were adopted.Results The average inhibitory rate of CHPG 125 ?g/mL was(29.8?2.3)%,(36.5?4.7)%and(30.3?8.8)%for HBsAg,HBeAg and HBV-DNA respectively.In the duckling hepatitis B model,CHPG 4.0,6.0,8.0 g/kg had an obvious effect in inhibiting the replication of DHBV-DNA,CHPG 8.0 g/kg in particular;the effect was weaker but maintained longer time than lamivudine.1.25,2.50,5.00 g/kg of CHPG could decreased serum levels of AST and ALT and protect liver cells mice with liver injury and increased serum hemolysin concentration(HC50).0.01～10 mg/L of CHPG accelerated the proliferation of T lymphocyte of mice in vitro.Conclusion CHPG is effective in reducing the secretion of HBsAg,HBeAg in vitro,inhibiting the replication of DHBV-DNA in duck hepatitis B model.It has effect in protecting liver cells,decreasing ALT and AST levels and regulating immune function.