Cervical tumor lysate-loaded dendritic cell induce the generation of lymphcyte / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)1986.
Article
in Chinese
| WPRIM
| ID: wpr-572539
ABSTRACT
Objective:
To make use of the characteristics of presenting and processing tumor antigen of dendritic cell to enhance generation of isotypical but xenogenous T lymphocyte.Methods:
Autologous dendritic cells were obtained by culturing peripheral blood monocyte of patients with cervical cancer in the presence of granulocyte-macrophage colony stimulating factor(GM-CSF) and interleukin-4(IL-4).Dendritic cells were loaded with tumor cell lysate and co-cultured with heterogenous PBMCs from patients to induce generation of tumor specific cytotoxic T cell.The generation rate was measured by MTT.Results:
Tumor lysate-loaded DC which was isolated from peripheral blood monocyte of patients with cervical cancer could induce the generation of isotypical but xenogenous T lymphcyte.There was no significant difference between tumor tissue lysate and tumor cell strain lysate(P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
1986
Type:
Article
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