Method study for determination of hesperidin in Citri reticulatae by HPLC / 中成药
Chinese Traditional Patent Medicine
;
(12)1992.
Article
in Chinese
| WPRIM
| ID: wpr-572614
ABSTRACT
AIM:
To establish a quantitative method for determination of hesperidin in Citri reticulatae in order to evaluate their quality.METHODS:
Sample was extracted with methanol by means of sonication for 30min. ODS column was used with methanol-water-acetic acid(35∶61∶2)as mobile phase. Detection wavelength was at 284nm.RESULTS:
Hesperidin in sample solution was well separated. Linearity was good(r=0.9995)in range of 0.202~2.424?g. The average recovery was 98%, RSD of repeatability was 2.3%.CONCLUSION:
This method is simple, accurate and reliable, which may well be used for the determination of hesperidin.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article
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