Determination of imperatorin in Qianzhi Capsules by RP-HPLC / 中成药

ABSTRACT

AIM:To determine imperatorin in Qianzhi Capsules(Radix Rubiae, Radix Angelicae Dahuricae, etc.). METHODS:RP-HPLC was applied. The mobile phase consisted of methanol 5% phosphoric acid solution(70∶30). The detection wavelength was at 248nm and the flow rate was 1.0mL?min -1. RESULTS:The linearity was obtained in the range of 12～60?g?mL -1(r=0.9998). The average recovery was 99.8% with relative standard deviation of 1.0%. CONCLUSION:The method was simple and reliable.