Therapeutic effect of adefovir anti-duck hepatitis B virus in vivo and anti-HBV activity in vitro (2.2.15 cells) / 重庆医科大学学报

ABSTRACT

Objective:To study the therapeutic effect of the purine nucleoside analog adefovir against duck hepatitis virus (DHBV) in vivo and anti-HBV activity in vitro.Methods:The Chongqing duck hepatitis B animal model was treated with adefovir by intragastric administration once a day for 10 days.DHBV DNA in serum and liver were monitored by serum dot-blot hybridization and Southern blot.DHBsAg in serum was detected simultaneously by ELISA.The anti-HBV activity of adefovir was observed in 2.2.15 cells (human hepatocellular carcinoma cells stably transfected with HBV).Results:Adefovir could significantly reduce DHBV DNA level.After stopping the treatment for 3 days,DHBV DNA level in serum and liver returned significantly.The O.D values of DHBsAg in duck serum did not change at the period of the treatment.After 12 days of continuous exposure to adefovir at the concentration of 20?g/ml in vitro culture,the inhibitions of HBsAg,HBeAg,HBV DNA were 17.01%,26.80%,and 26.92% respectively.Conclusion:The study confirms the safety and potent dose-dependent antihepadnaviral activity of adefovir in vivo.The anti-HBV effect is not demonstrated in 2.2.15 cells.