Effects of environment in vivo and in vitro on the differentiation of bone mesenchymal stem cells into neural cells / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2003.
Article
in Chinese
| WPRIM
| ID: wpr-572737
ABSTRACT
Objective:
To investigate the effects of environment in vitro and in vivo on the differentiation of bone mesenchymal stem cells (MSCs) into neural cells.Methods:
①MSCs were labeled with 4,6-diamidino-2-phenylindole (DAPI) and injected into the right brain of the rats(n=6),which had suffered from middle cerebral artery occlusion(MACO) a day before.Two weeks later,immunofluorescent staining was used to detect the expression of nestin,microtubule-associated protein 2 (MAP-2) and glial fibrillary acidic protein (GFAP).②In vitro,MSCs were cultured with modified neuronal medium(MNM)for 18 hours after being pretreated with 0.5umol/L all-trans retinoid acid (ATRA) for 24 hours.Immunocytochemistry was used to detect the expression of nestin,MAP-2 and GFAP.Results:
①After being injected into rats for 2 weeks,MSCs survived in the ischemic areas and a few cells expressed nestin,MAP-2 and GFAP respectively.②In vitro,after ATRA and MNM treatment,the positive percentage of nestin and MAP-2 were (92.3?3.4)% and (89.6?3.3)% respectively,but no expression of GFAP was detected.Conclusion:
The different ratio of MSCs differentiating into neural cells suggests that there is difference in the process and mechanism between environment in vitro and in vivo.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
2003
Type:
Article
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