The study on expression of nerve growth factor and its receptor in the rat catha rtic colon / 中华消化杂志

ABSTRACT

Objective To investigate the action and the mechanisms of nerve growth factor(NGF) and its receptor(p75、TrkA) on the pathological alterations of the enteric nervous syst em in the rats cathartic colon. Methods The experimental animal model of cathartic colon was established by using rhubar b and phenolphthalein. Thirty Sprague Dawley (SD) rats were randomly divided int o the control group(10 rats) and cathartic colon model groups(10 rats with rhuba r b group; 10 rats with phenolphthalein group). The model group rats were fed with stimulant laxatives rhubarb and phenolphthalein respectively, and the control g roup rats were fed with distilled water. The rats were killed and the colon samp les were taken after 3 months. The expressions of the mRNA of NGF, p75 and TrkA in the colonic tissues were detected by semi-quantitive reverse transcription- polymerase chain reaction(RT-PCR) technique. Results The expressional levels of NGF mRNA were lower in the rhubarb group and phenolph thalein group than that in control group (P