Determination of astragaloside in Yiganning Granules by HPLC-ELSD / 中成药

ABSTRACT

AIM: To establish a HPLC-ELSD method for determination of astragaloside in Yiganning Granules. METHODS: The HPLC-ELSD with the Agilent C 18 column (4.6 mm?250 mm, 5 ?m) was used. The mobile phase was methanol-water (75∶25) at a flow rate of 1.0 mm?min -1 and the detector was SEDEX75. RESULTS: There was a good linear correlation in the range of 0.5984-2.3936?g. The recovery was 95.41% (RSD=1.1%, n=5). CONCLUSION: The method is proved to be precise、reproducibility and reliable. It is suitable for determination of astragaloside.